Pdonr201

Plasmid Type: Other, Gateway Donor vector. Cloning Method: Gateway Cloning. Synonyms: Type: bacterial plasmid. DONR2has been discontinued by Invitrogen.

B Ttranscription termination sequence (c):.

B Ttranscription termination sequence (c):. Er is geen beschrijving beschikbaar voor dit resultaat vanwege de robots. Step 2: Construct an Expression Clone.

Choose or Construct your Destination Vector. Description, Gateway(TM)-adapted entry vector designed to generate . BP products are then transformed into E. DH5-alpha strain and plated in .

Selection marker: Kanamycin. L1-MCS-attR4-RCS-Ampr-attR3-attL2. DONR 2Print this window. GGGGACAAGTTTGTACAAAAAAGCAGGCTTCATGTCG.

Additional sequence has been added in front of the start codon: att . CTTTTCGGTACAAGTACCACGGCT. BTtranscription termination sequence. BTtranscription termination sequence. M13F, 5-GTAAAACGACGGCCAGT-3.

CAT CAG AGA TTT TGA GAC AC. GCA GTT CCC TAC TCT CGC. DNA pCEP pCI, pCMV, pCMV pCMV-Sport pCRII, pCR2. QdtC_rev CGTTATACAAAATTCTTCCCCC. BCKS II, Chromosome walking, Stratagene.

SeqL-A( proximal to attL1).

TCGCGTTAACGCTAGCATGGATCTC. PCR product for recombination. Add reagents in the following order: uL Ligation Reaction 1. L BP Reaction Buffer (5X). Currently, I have prepared the DB3. And the kanamycin was used as .

More from my site